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What is peroxide value in seed oils?

Short answer

Peroxide value measures the hydroperoxides formed when oxygen attacks the unsaturated fatty acids in an oil. It is determined by ISO 3960 and reported in milliequivalents of active oxygen per kilogram of oil. It is the standard first indicator of oxidative deterioration in a seed oil, and it is routinely misread.

Why it works this way

Oxidation runs in two stages. Hydroperoxides form first, which is what the test detects, and then break down into secondary products, the aldehydes and ketones that produce the rancid smell. Because the second stage consumes what the first stage produced, the peroxide value rises, peaks and then falls. A low figure therefore means either a fresh oil or a badly degraded one, and the number alone cannot distinguish them.

Two other properties make it fragile as a contractual parameter. It is sensitive to how the sample was drawn, stored and transported before testing, so a delayed sample gives a higher result than the oil deserves. And it describes a moment rather than a trajectory: an oil with a low peroxide value and no remaining antioxidant capacity will fail faster than one with a slightly higher value and intact tocopherols.

The companion parameter is acid value, or free fatty acid content, determined by ISO 660. It measures hydrolytic rather than oxidative breakdown and largely reports on seed quality and on the interval before pressing. Free acidity and peroxide value together describe an oil far better than either alone.

What follows in practice

Specify peroxide value with the method, the maximum at delivery rather than only at production, and the production date on the certificate. Where oxidative stability drives shelf life, add an accelerated stability test such as a Rancimat induction time. Cold-pressed seed oils sets out the full parameter set, and how to store cold-pressed oils covers what happens after delivery.

Sources & References

  • ISO 3960 Animal and vegetable fats and oils – Determination of peroxide value – Iodometric (visual) endpoint determinationInternational Organization for Standardization · accessed August 22, 2026The reference method behind the peroxide value on an oil or a high-fat kernel certificate. It measures primary oxidation products, which rise and then fall as those products break down, so the figure is only interpretable next to a production date and a temperature history. Sample handling between drawing and testing moves the result, which is why the measurement point belongs in the contract.
  • ISO 660 Animal and vegetable fats and oils – Determination of acid value and acidityInternational Organization for Standardization · accessed August 22, 2026Free fatty acid content measures hydrolytic rather than oxidative breakdown, and it reports mainly on the condition of the seed and on the interval between harvest and pressing. Read together with peroxide value it separates a raw material problem from a storage problem, which is the distinction a rejection conversation turns on.
  • Codex Standard for Named Vegetable Oils (CXS 210-1999, revised)FAO and WHO, Codex Alimentarius Commission · accessed August 22, 2026Defines each named oil by fatty acid range and sets the quality and identity characteristics – acid value, peroxide value, refractive index, unsaponifiable matter. This is the document that decides whether an oil may be sold as sunflower oil at all, and the fatty acid table is what distinguishes standard from high oleic.

Evidence confidence: partial

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Vorezan publishes reference information for buyers and suppliers. We are not a certification body, a customs broker or a guarantor of any third party. Regulatory references point to the framework in force at the review date; verify the current consolidated text and your own obligations before relying on them commercially.

Last updated: August 22, 2026Sources & references

About this Article

  • Topiclab-testing, oilseed-processing
  • UpdatedAugust 22, 2026

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